20. Investigation into the separation of chloroplast pigments by chromatography
- 00:36 Why do you grind the leaves with sand?
- 01:05 Why must you use a pencil and not a pen for the line?
- 01:28 Why do you need to concentrate the pigment?
- 01:50 Why must the chromatography paper touch the solvent?
- 02:20 Why must the pigment spot be above the solvent surface?
- 02:30 Which pigments can you identify?
dark green leaves e.g. spinach, stinging nettle
chromatography paper or silica gel chromatography plates
pestle and mortar
2 x boiling tube
2 x stopper
Propanone: Highly flammable liquid and vapour Can cause serious eye irritation
Contact with naked flame Contact with eyes and skin
Ensure no naked flames or other sources of ignition. Ensure laboratory is well-ventilated. Eye: flood with tap water (10min)
Petroleum ether: Highly flammable liquid and vapour
Contact with naked flame
Ensure no naked flames or other sources of ignition. Ensure laboratory is well-ventilated.
Cuts to skin
Cut away from body