Biology

19. Investigation of dehydrogenase activity in yeast

  • 00:22 Which molecules act as a hydrogen acceptors during aerobic respiration?
  • 00:29 How will you adapt this method to investigate dehydrogenase activity in yeast?

  • redox indicator: methylene blue (0.05g/100cm3)

  • yeast suspension (100g/dm-3)

  • 30oC Water bath

  • Test tube

  • Cork for test tube

  • 10 cm3 syringe

  • 1 cm3 syringe

  • Stop clock

Hazard Risk Control measure

Methylene blue - irritant to eyes

Contact with eyes

Eye: flood with tap water (10min)

Yeast suspension - irritant to eyes

Contact with eyes

Eye: flood with tap water (10min)