Biology
10. Scientific drawing of cells from slides of root tip to show stages of mitosis
- 00:39 Which molecules are stained red with acetic-orcein stain?
- 01:15 Why do you need to squash the root tip?
- 01:26 What stages of mitosis can you identify on your slide?
- 01:34 Which is the most common stage of mitosis you can see?
- 01:34 What does this tell you about the relative length of time cells spend in this phase?
microscope
bunsen burner / hot plate
garlic or onion with developing roots
microscope slide and cover slip
scalpel
fine forceps
watch glass
mounted needle
acetic-orcein stain
dropping pipette
1M hydrochloric acid
paper towel
| Hazard | Risk | Control measure |
|---|---|---|
Acetic-orcein stain - Irritant to skin and eyes |
Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min) Avoid inhaling vapour. |
1M hydrochloric acid - Irritant to skin and eyes |
Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min) |
Scalpel |
Cuts to skin |
Cut down and away from body onto a white tile or chopping board |
Bunsen burner/hotplate |
Risk of burn or cracking slide |
Hold slide with forceps Only heat slide for 4-5s Do not touch the hotplate |
Mounted needle – sharp point |
Risk of pricking skin |
Carry pointing downwards, away from body |