Biology
08. Investigation into the effect of enzyme or substrate concentration on enzyme activity
- 00:24 Why do you have to grind the potato cells?
- 00:54 How would cell debris on the disc affect the rise time?
- 01:00 What gas is given off by this reaction?
- 01:03 How does this gas affect the density of the paper disc?
- 01:03 What effect does this density change have on the disc rise time?
- 01:05 How can you use this method to investigate the effect of enzyme concentration (mass of potato used) or substrate (H2O2) concentration on enzyme activity?
freshly cut potato cylinders
pestle and mortar
specimen tubes / test tubes
stock solution of hydrogen peroxide
filter paper discs
forceps
stopwatch
syringe
deionised water
paper towel
| Hazard | Risk | Control measure |
|---|---|---|
Hydrogen peroxide stock solution – irritant to eyes and skin |
Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min) |
Potato enzyme paste – possible allergen |
Enzymes may produce allergic reactions. Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min) |