Biology

01. Food Tests

Test for reducing sugars

  • 00:51 What is the colour of the mixture at the start?
  • 01:06 What do you observe in the tube after heating for 5 minutes?

Test for non-reducing sugars

  • 01:10 What is the colour of the mixture at the start?
  • 01:26 What do you observe in the tube after heating for 5 minutes?
  • 01:28 Why do you add dilute hydrochloric acid to the non-reducing sugar and heat it?
  • 01:44 Why do you then add dilute sodium hydroxide or sodium bicarbonate?
  • 01:51 What do you observe?
  • 01:58 Can you explain why the Benedict’s test was negative at first, and then positive after adding acid, heating and adding alkali?

Test for protein

  • 02:06 What colour is Biuret reagent at the start?
  • 02:23 What colour is the solution after adding Biuret reagent?

Test for starch

  • 02:26 What colour is iodine-potassium iodide solution at the start?
  • 02:35 What colour is iodine-potassium iodide solution in the presence of starch?

Test for fats and oils

  • 02:46 What do you observe where the alcohol and oil meet?
  • 5 boiling tubes

  • 2 test tubes

  • 1 beaker

  • 3 dropping pipettes

  • Benedict's reagent

  • dilute hydrochloric acid (0.5 moldm-3)

  • dilute sodium hydroxide solution/sodium bicarbonate

  • Biuret reagent

  • iodine-potassium iodide solution

  • absolute alcohol

  • glucose solution

  • sucrose solution

  • albumen solution

  • starch solution

  • oil

  • water bath

Hazard Risk Control measure

Benedict’s reagent - irritant

Contact with eyes and skin

Eye: flood with tap water (10min)

Skin: drench with water.

Flood with tap water (10min)

Biuret reagent - irritant

Contact with eyes and skin

Eye: flood with tap water (10min)

Skin: drench with water.

Flood with tap water (10min)

Iodine-potassium

iodide solution - irritant

Contact with eyes and skin

Eye: flood with tap water (10min)

Skin: drench with water.

Flood with tap water (10min)

Absolute alcohol - flammable

Contact with eyes Contact with naked flame

Eye: flood with tap water (10min)

Ensure no naked flames or other sources of ignition.

Dilute hydrochloric acid - irritant

Contact with eyes and skin

Eye: flood with tap water (10min)

Skin: drench with water.

Flood with tap water (10min)

Dilute sodium hydroxide - irritant

Contact with eyes and skin

Eye: flood with tap water (10min)

Skin: drench with water.

Flood with tap water (10min).