01. Food Tests
Test for reducing sugars
- 00:51 What is the colour of the mixture at the start?
- 01:06 What do you observe in the tube after heating for 5 minutes?
Test for non-reducing sugars
- 01:10 What is the colour of the mixture at the start?
- 01:26 What do you observe in the tube after heating for 5 minutes?
- 01:28 Why do you add dilute hydrochloric acid to the non-reducing sugar and heat it?
- 01:44 Why do you then add dilute sodium hydroxide or sodium bicarbonate?
- 01:51 What do you observe?
- 01:58 Can you explain why the Benedict’s test was negative at first, and then positive after adding acid, heating and adding alkali?
Test for protein
- 02:06 What colour is Biuret reagent at the start?
- 02:23 What colour is the solution after adding Biuret reagent?
Test for starch
- 02:26 What colour is iodine-potassium iodide solution at the start?
- 02:35 What colour is iodine-potassium iodide solution in the presence of starch?
Test for fats and oils
- 02:46 What do you observe where the alcohol and oil meet?
5 boiling tubes
2 test tubes
1 beaker
3 dropping pipettes
Benedict's reagent
dilute hydrochloric acid (0.5 moldm-3)
dilute sodium hydroxide solution/sodium bicarbonate
Biuret reagent
iodine-potassium iodide solution
absolute alcohol
glucose solution
sucrose solution
albumen solution
starch solution
oil
water bath
Hazard | Risk | Control measure |
---|---|---|
Benedict’s reagent - irritant |
Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min) |
Biuret reagent - irritant |
Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min) |
Iodine-potassium iodide solution - irritant |
Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min) |
Absolute alcohol - flammable |
Contact with eyes Contact with naked flame |
Eye: flood with tap water (10min) Ensure no naked flames or other sources of ignition. |
Dilute hydrochloric acid - irritant |
Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min) |
Dilute sodium hydroxide - irritant |
Contact with eyes and skin |
Eye: flood with tap water (10min) Skin: drench with water. Flood with tap water (10min). |